pet-28 b (+) vector (Millipore)
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pet-28 b (+) vector
Pet 28 B (+) Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-28(b)+vector/pet28a/pm33247466-61-8-12
Average 90 stars, based on 1 article reviews
Pet 28 B (+) Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-28(b)+vector/pet28a/pm33247466-61-8-12
Average 90 stars, based on 1 article reviews
pet-28 b (+) vector - by Bioz Stars,
2026-10
90/100 stars
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Plasmid Preparation:Article Title: DNA-Mediated Signaling by Proteins with 4Fe–4S Clusters Is Necessary for Genomic Integrity Article Snippet: The dinG gene was amplified from E. coli and was inserted into a Article Title: Regulation of gene expression using chromatin remodelling factors Article Snippet: Generation of Article Title: Delineating the reaction mechanism of reductase domains of Nonribosomal Peptide Synthetases from mycobacteria. Article Snippet: Substrate binding to enzymes often follows a precise order where catalysis is accomplished through programmed conformational changes.. Short-chain dehydrogenase/reductase (SDR) enzymes follow sequential order ‘bi–bi’ reaction kinetics.. The mechanistic study of a SDR homolog, reductase (R) domain, from multifunctional enzymes, e.g. Nonribosomal Peptide Synthetases (NRPSs) and Polyketide Synthases (PKSs) has revealed that it reductively releases 40-phosphopantetheinyl arm-tethered peptidyl product. Article Title: Structural insights into the regulation of NADPH binding to reductase domains of nonribosomal peptide synthetases: A concerted loop movement model. Article Snippet: The termination module of nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) offloads the final product as an acid (occasionally also accompanied by cyclization) upon hydrolysis by employing thioesterase domains (TE-domains).. Reductase domains (R-domains) of short-chain dehydrogenase/reductase (SDR) family offer an alternative offloading mechanism by reducing 4′-phosphopantetheine (4′-PPant) arm-tethered peptidyl chain, a thioester, to an aldehyde or an alcohol.. Recent studies have highlighted their functional importance, for instance in the glycopeptidolipid (GPL) biosynthesis of Mycobacterium smegmatis, where the resulting alcoholic group is the site for subsequent modifications such as glycosylations. Article Title: The heterologous expression of a plastocyanin in the diatom Phaeodactylum tricornutum improves cell growth under iron-deficient conditions. Article Snippet: The PCR product was then cloned into the Article Title: Recombinant expression, purification, and crystallization of the glutaminyl-tRNA synthetase from Toxoplasma gondii. Article Snippet: Aminoacyl tRNA synthetases play a critical role in protein synthesis by providing precursor transfer-RNA molecules correctly charged with their cognate amino-acids.. The essential nature of these enzymes make them attractive targets for designing new drugs against important pathogenic protozoans like Toxoplasma.. Because no structural data currently exists for a protozoan glutaminyl-tRNA synthetase (QRS), an understanding of its potential as a drug target and its function in the assembly of the Toxoplasma multi-aminoacyl tRNA (MARS) complex is therefore lacking. Article Title: Upstream sequence-dependent suppression and AtxA-dependent activation of protective antigens in Bacillus anthracis Article Snippet: To examine the interaction between AtxA and DNA sequences in vitro, the atxA gene was cloned into the expression vector pET 28-b (Novagen, Madison, WI, USA) with N- and C-terminal 6xHIS-tags and an internal FLAG-tag. Article Title: Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs Article Snippet: The LT-A2/LT-B (referred to as LTA2B) fragment from the plasmid pEnt [ ] was PCR amplified and cloned into an N-terminus His 6 tag containing expression vector, Polymerase Chain Reaction:Article Title: DNA-Mediated Signaling by Proteins with 4Fe–4S Clusters Is Necessary for Genomic Integrity Article Snippet: The dinG gene was amplified from E. coli and was inserted into a Article Title: Regulation of gene expression using chromatin remodelling factors Article Snippet: Generation of Article Title: Delineating the reaction mechanism of reductase domains of Nonribosomal Peptide Synthetases from mycobacteria. Article Snippet: Substrate binding to enzymes often follows a precise order where catalysis is accomplished through programmed conformational changes.. Short-chain dehydrogenase/reductase (SDR) enzymes follow sequential order ‘bi–bi’ reaction kinetics.. The mechanistic study of a SDR homolog, reductase (R) domain, from multifunctional enzymes, e.g. Nonribosomal Peptide Synthetases (NRPSs) and Polyketide Synthases (PKSs) has revealed that it reductively releases 40-phosphopantetheinyl arm-tethered peptidyl product. Article Title: Structural insights into the regulation of NADPH binding to reductase domains of nonribosomal peptide synthetases: A concerted loop movement model. Article Snippet: The termination module of nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) offloads the final product as an acid (occasionally also accompanied by cyclization) upon hydrolysis by employing thioesterase domains (TE-domains).. Reductase domains (R-domains) of short-chain dehydrogenase/reductase (SDR) family offer an alternative offloading mechanism by reducing 4′-phosphopantetheine (4′-PPant) arm-tethered peptidyl chain, a thioester, to an aldehyde or an alcohol.. Recent studies have highlighted their functional importance, for instance in the glycopeptidolipid (GPL) biosynthesis of Mycobacterium smegmatis, where the resulting alcoholic group is the site for subsequent modifications such as glycosylations. Article Title: The heterologous expression of a plastocyanin in the diatom Phaeodactylum tricornutum improves cell growth under iron-deficient conditions. Article Snippet: The PCR product was then cloned into the Article Title: Recombinant expression, purification, and crystallization of the glutaminyl-tRNA synthetase from Toxoplasma gondii. Article Snippet: Aminoacyl tRNA synthetases play a critical role in protein synthesis by providing precursor transfer-RNA molecules correctly charged with their cognate amino-acids.. The essential nature of these enzymes make them attractive targets for designing new drugs against important pathogenic protozoans like Toxoplasma.. Because no structural data currently exists for a protozoan glutaminyl-tRNA synthetase (QRS), an understanding of its potential as a drug target and its function in the assembly of the Toxoplasma multi-aminoacyl tRNA (MARS) complex is therefore lacking. Article Title: Upstream sequence-dependent suppression and AtxA-dependent activation of protective antigens in Bacillus anthracis Article Snippet: To examine the interaction between AtxA and DNA sequences in vitro, the atxA gene was cloned into the expression vector pET 28-b (Novagen, Madison, WI, USA) with N- and C-terminal 6xHIS-tags and an internal FLAG-tag. Article Title: Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs Article Snippet: The LT-A2/LT-B (referred to as LTA2B) fragment from the plasmid pEnt [ ] was PCR amplified and cloned into an N-terminus His 6 tag containing expression vector, Amplification:Article Title: DNA-Mediated Signaling by Proteins with 4Fe–4S Clusters Is Necessary for Genomic Integrity Article Snippet: The dinG gene was amplified from E. coli and was inserted into a Article Title: Regulation of gene expression using chromatin remodelling factors Article Snippet: Generation of Article Title: Delineating the reaction mechanism of reductase domains of Nonribosomal Peptide Synthetases from mycobacteria. Article Snippet: Substrate binding to enzymes often follows a precise order where catalysis is accomplished through programmed conformational changes.. Short-chain dehydrogenase/reductase (SDR) enzymes follow sequential order ‘bi–bi’ reaction kinetics.. The mechanistic study of a SDR homolog, reductase (R) domain, from multifunctional enzymes, e.g. Nonribosomal Peptide Synthetases (NRPSs) and Polyketide Synthases (PKSs) has revealed that it reductively releases 40-phosphopantetheinyl arm-tethered peptidyl product. Article Title: Structural insights into the regulation of NADPH binding to reductase domains of nonribosomal peptide synthetases: A concerted loop movement model. Article Snippet: The termination module of nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) offloads the final product as an acid (occasionally also accompanied by cyclization) upon hydrolysis by employing thioesterase domains (TE-domains).. Reductase domains (R-domains) of short-chain dehydrogenase/reductase (SDR) family offer an alternative offloading mechanism by reducing 4′-phosphopantetheine (4′-PPant) arm-tethered peptidyl chain, a thioester, to an aldehyde or an alcohol.. Recent studies have highlighted their functional importance, for instance in the glycopeptidolipid (GPL) biosynthesis of Mycobacterium smegmatis, where the resulting alcoholic group is the site for subsequent modifications such as glycosylations. Article Title: The heterologous expression of a plastocyanin in the diatom Phaeodactylum tricornutum improves cell growth under iron-deficient conditions. Article Snippet: The PCR product was then cloned into the Article Title: Recombinant expression, purification, and crystallization of the glutaminyl-tRNA synthetase from Toxoplasma gondii. Article Snippet: Aminoacyl tRNA synthetases play a critical role in protein synthesis by providing precursor transfer-RNA molecules correctly charged with their cognate amino-acids.. The essential nature of these enzymes make them attractive targets for designing new drugs against important pathogenic protozoans like Toxoplasma.. Because no structural data currently exists for a protozoan glutaminyl-tRNA synthetase (QRS), an understanding of its potential as a drug target and its function in the assembly of the Toxoplasma multi-aminoacyl tRNA (MARS) complex is therefore lacking. Article Title: Upstream sequence-dependent suppression and AtxA-dependent activation of protective antigens in Bacillus anthracis Article Snippet: To examine the interaction between AtxA and DNA sequences in vitro, the atxA gene was cloned into the expression vector pET 28-b (Novagen, Madison, WI, USA) with N- and C-terminal 6xHIS-tags and an internal FLAG-tag. Article Title: Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs Article Snippet: The LT-A2/LT-B (referred to as LTA2B) fragment from the plasmid pEnt [ ] was PCR amplified and cloned into an N-terminus His 6 tag containing expression vector, Clone Assay:Article Title: DNA-Mediated Signaling by Proteins with 4Fe–4S Clusters Is Necessary for Genomic Integrity Article Snippet: The dinG gene was amplified from E. coli and was inserted into a Article Title: Regulation of gene expression using chromatin remodelling factors Article Snippet: Generation of Article Title: Delineating the reaction mechanism of reductase domains of Nonribosomal Peptide Synthetases from mycobacteria. Article Snippet: Substrate binding to enzymes often follows a precise order where catalysis is accomplished through programmed conformational changes.. Short-chain dehydrogenase/reductase (SDR) enzymes follow sequential order ‘bi–bi’ reaction kinetics.. The mechanistic study of a SDR homolog, reductase (R) domain, from multifunctional enzymes, e.g. Nonribosomal Peptide Synthetases (NRPSs) and Polyketide Synthases (PKSs) has revealed that it reductively releases 40-phosphopantetheinyl arm-tethered peptidyl product. Article Title: Structural insights into the regulation of NADPH binding to reductase domains of nonribosomal peptide synthetases: A concerted loop movement model. Article Snippet: The termination module of nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) offloads the final product as an acid (occasionally also accompanied by cyclization) upon hydrolysis by employing thioesterase domains (TE-domains).. Reductase domains (R-domains) of short-chain dehydrogenase/reductase (SDR) family offer an alternative offloading mechanism by reducing 4′-phosphopantetheine (4′-PPant) arm-tethered peptidyl chain, a thioester, to an aldehyde or an alcohol.. Recent studies have highlighted their functional importance, for instance in the glycopeptidolipid (GPL) biosynthesis of Mycobacterium smegmatis, where the resulting alcoholic group is the site for subsequent modifications such as glycosylations. Article Title: The heterologous expression of a plastocyanin in the diatom Phaeodactylum tricornutum improves cell growth under iron-deficient conditions. Article Snippet: The PCR product was then cloned into the Article Title: Recombinant expression, purification, and crystallization of the glutaminyl-tRNA synthetase from Toxoplasma gondii. Article Snippet: Aminoacyl tRNA synthetases play a critical role in protein synthesis by providing precursor transfer-RNA molecules correctly charged with their cognate amino-acids.. The essential nature of these enzymes make them attractive targets for designing new drugs against important pathogenic protozoans like Toxoplasma.. Because no structural data currently exists for a protozoan glutaminyl-tRNA synthetase (QRS), an understanding of its potential as a drug target and its function in the assembly of the Toxoplasma multi-aminoacyl tRNA (MARS) complex is therefore lacking. Article Title: Upstream sequence-dependent suppression and AtxA-dependent activation of protective antigens in Bacillus anthracis Article Snippet: To examine the interaction between AtxA and DNA sequences in vitro, the atxA gene was cloned into the expression vector pET 28-b (Novagen, Madison, WI, USA) with N- and C-terminal 6xHIS-tags and an internal FLAG-tag. Article Title: Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs Article Snippet: The LT-A2/LT-B (referred to as LTA2B) fragment from the plasmid pEnt [ ] was PCR amplified and cloned into an N-terminus His 6 tag containing expression vector, Expressing:Article Title: DNA-Mediated Signaling by Proteins with 4Fe–4S Clusters Is Necessary for Genomic Integrity Article Snippet: The dinG gene was amplified from E. coli and was inserted into a Article Title: Regulation of gene expression using chromatin remodelling factors Article Snippet: Generation of Article Title: Delineating the reaction mechanism of reductase domains of Nonribosomal Peptide Synthetases from mycobacteria. Article Snippet: Substrate binding to enzymes often follows a precise order where catalysis is accomplished through programmed conformational changes.. Short-chain dehydrogenase/reductase (SDR) enzymes follow sequential order ‘bi–bi’ reaction kinetics.. The mechanistic study of a SDR homolog, reductase (R) domain, from multifunctional enzymes, e.g. Nonribosomal Peptide Synthetases (NRPSs) and Polyketide Synthases (PKSs) has revealed that it reductively releases 40-phosphopantetheinyl arm-tethered peptidyl product. Article Title: Structural insights into the regulation of NADPH binding to reductase domains of nonribosomal peptide synthetases: A concerted loop movement model. Article Snippet: The termination module of nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) offloads the final product as an acid (occasionally also accompanied by cyclization) upon hydrolysis by employing thioesterase domains (TE-domains).. Reductase domains (R-domains) of short-chain dehydrogenase/reductase (SDR) family offer an alternative offloading mechanism by reducing 4′-phosphopantetheine (4′-PPant) arm-tethered peptidyl chain, a thioester, to an aldehyde or an alcohol.. Recent studies have highlighted their functional importance, for instance in the glycopeptidolipid (GPL) biosynthesis of Mycobacterium smegmatis, where the resulting alcoholic group is the site for subsequent modifications such as glycosylations. Article Title: The heterologous expression of a plastocyanin in the diatom Phaeodactylum tricornutum improves cell growth under iron-deficient conditions. Article Snippet: The PCR product was then cloned into the Article Title: Recombinant expression, purification, and crystallization of the glutaminyl-tRNA synthetase from Toxoplasma gondii. Article Snippet: Aminoacyl tRNA synthetases play a critical role in protein synthesis by providing precursor transfer-RNA molecules correctly charged with their cognate amino-acids.. The essential nature of these enzymes make them attractive targets for designing new drugs against important pathogenic protozoans like Toxoplasma.. Because no structural data currently exists for a protozoan glutaminyl-tRNA synthetase (QRS), an understanding of its potential as a drug target and its function in the assembly of the Toxoplasma multi-aminoacyl tRNA (MARS) complex is therefore lacking. Article Title: Upstream sequence-dependent suppression and AtxA-dependent activation of protective antigens in Bacillus anthracis Article Snippet: To examine the interaction between AtxA and DNA sequences in vitro, the atxA gene was cloned into the expression vector pET 28-b (Novagen, Madison, WI, USA) with N- and C-terminal 6xHIS-tags and an internal FLAG-tag. Article Title: Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs Article Snippet: The LT-A2/LT-B (referred to as LTA2B) fragment from the plasmid pEnt [ ] was PCR amplified and cloned into an N-terminus His 6 tag containing expression vector, In Vitro:Article Title: DNA-Mediated Signaling by Proteins with 4Fe–4S Clusters Is Necessary for Genomic Integrity Article Snippet: The dinG gene was amplified from E. coli and was inserted into a Article Title: Regulation of gene expression using chromatin remodelling factors Article Snippet: Generation of Article Title: Delineating the reaction mechanism of reductase domains of Nonribosomal Peptide Synthetases from mycobacteria. Article Snippet: Substrate binding to enzymes often follows a precise order where catalysis is accomplished through programmed conformational changes.. Short-chain dehydrogenase/reductase (SDR) enzymes follow sequential order ‘bi–bi’ reaction kinetics.. The mechanistic study of a SDR homolog, reductase (R) domain, from multifunctional enzymes, e.g. Nonribosomal Peptide Synthetases (NRPSs) and Polyketide Synthases (PKSs) has revealed that it reductively releases 40-phosphopantetheinyl arm-tethered peptidyl product. Article Title: Structural insights into the regulation of NADPH binding to reductase domains of nonribosomal peptide synthetases: A concerted loop movement model. Article Snippet: The termination module of nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) offloads the final product as an acid (occasionally also accompanied by cyclization) upon hydrolysis by employing thioesterase domains (TE-domains).. Reductase domains (R-domains) of short-chain dehydrogenase/reductase (SDR) family offer an alternative offloading mechanism by reducing 4′-phosphopantetheine (4′-PPant) arm-tethered peptidyl chain, a thioester, to an aldehyde or an alcohol.. Recent studies have highlighted their functional importance, for instance in the glycopeptidolipid (GPL) biosynthesis of Mycobacterium smegmatis, where the resulting alcoholic group is the site for subsequent modifications such as glycosylations. Article Title: The heterologous expression of a plastocyanin in the diatom Phaeodactylum tricornutum improves cell growth under iron-deficient conditions. Article Snippet: The PCR product was then cloned into the Article Title: Recombinant expression, purification, and crystallization of the glutaminyl-tRNA synthetase from Toxoplasma gondii. Article Snippet: Aminoacyl tRNA synthetases play a critical role in protein synthesis by providing precursor transfer-RNA molecules correctly charged with their cognate amino-acids.. The essential nature of these enzymes make them attractive targets for designing new drugs against important pathogenic protozoans like Toxoplasma.. Because no structural data currently exists for a protozoan glutaminyl-tRNA synthetase (QRS), an understanding of its potential as a drug target and its function in the assembly of the Toxoplasma multi-aminoacyl tRNA (MARS) complex is therefore lacking. Article Title: Upstream sequence-dependent suppression and AtxA-dependent activation of protective antigens in Bacillus anthracis Article Snippet: To examine the interaction between AtxA and DNA sequences in vitro, the atxA gene was cloned into the expression vector pET 28-b (Novagen, Madison, WI, USA) with N- and C-terminal 6xHIS-tags and an internal FLAG-tag. Article Title: Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs Article Snippet: The LT-A2/LT-B (referred to as LTA2B) fragment from the plasmid pEnt [ ] was PCR amplified and cloned into an N-terminus His 6 tag containing expression vector, Protein Purification:Article Title: DNA-Mediated Signaling by Proteins with 4Fe–4S Clusters Is Necessary for Genomic Integrity Article Snippet: The dinG gene was amplified from E. coli and was inserted into a Article Title: Regulation of gene expression using chromatin remodelling factors Article Snippet: Generation of Article Title: Delineating the reaction mechanism of reductase domains of Nonribosomal Peptide Synthetases from mycobacteria. Article Snippet: Substrate binding to enzymes often follows a precise order where catalysis is accomplished through programmed conformational changes.. Short-chain dehydrogenase/reductase (SDR) enzymes follow sequential order ‘bi–bi’ reaction kinetics.. The mechanistic study of a SDR homolog, reductase (R) domain, from multifunctional enzymes, e.g. Nonribosomal Peptide Synthetases (NRPSs) and Polyketide Synthases (PKSs) has revealed that it reductively releases 40-phosphopantetheinyl arm-tethered peptidyl product. Article Title: Structural insights into the regulation of NADPH binding to reductase domains of nonribosomal peptide synthetases: A concerted loop movement model. Article Snippet: The termination module of nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) offloads the final product as an acid (occasionally also accompanied by cyclization) upon hydrolysis by employing thioesterase domains (TE-domains).. Reductase domains (R-domains) of short-chain dehydrogenase/reductase (SDR) family offer an alternative offloading mechanism by reducing 4′-phosphopantetheine (4′-PPant) arm-tethered peptidyl chain, a thioester, to an aldehyde or an alcohol.. Recent studies have highlighted their functional importance, for instance in the glycopeptidolipid (GPL) biosynthesis of Mycobacterium smegmatis, where the resulting alcoholic group is the site for subsequent modifications such as glycosylations. Article Title: The heterologous expression of a plastocyanin in the diatom Phaeodactylum tricornutum improves cell growth under iron-deficient conditions. Article Snippet: The PCR product was then cloned into the Article Title: Recombinant expression, purification, and crystallization of the glutaminyl-tRNA synthetase from Toxoplasma gondii. Article Snippet: Aminoacyl tRNA synthetases play a critical role in protein synthesis by providing precursor transfer-RNA molecules correctly charged with their cognate amino-acids.. The essential nature of these enzymes make them attractive targets for designing new drugs against important pathogenic protozoans like Toxoplasma.. Because no structural data currently exists for a protozoan glutaminyl-tRNA synthetase (QRS), an understanding of its potential as a drug target and its function in the assembly of the Toxoplasma multi-aminoacyl tRNA (MARS) complex is therefore lacking. Article Title: Upstream sequence-dependent suppression and AtxA-dependent activation of protective antigens in Bacillus anthracis Article Snippet: To examine the interaction between AtxA and DNA sequences in vitro, the atxA gene was cloned into the expression vector pET 28-b (Novagen, Madison, WI, USA) with N- and C-terminal 6xHIS-tags and an internal FLAG-tag. Article Title: Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs Article Snippet: The LT-A2/LT-B (referred to as LTA2B) fragment from the plasmid pEnt [ ] was PCR amplified and cloned into an N-terminus His 6 tag containing expression vector, Centrifugation:Article Title: DNA-Mediated Signaling by Proteins with 4Fe–4S Clusters Is Necessary for Genomic Integrity Article Snippet: The dinG gene was amplified from E. coli and was inserted into a Article Title: Regulation of gene expression using chromatin remodelling factors Article Snippet: Generation of Article Title: Delineating the reaction mechanism of reductase domains of Nonribosomal Peptide Synthetases from mycobacteria. Article Snippet: Substrate binding to enzymes often follows a precise order where catalysis is accomplished through programmed conformational changes.. Short-chain dehydrogenase/reductase (SDR) enzymes follow sequential order ‘bi–bi’ reaction kinetics.. The mechanistic study of a SDR homolog, reductase (R) domain, from multifunctional enzymes, e.g. Nonribosomal Peptide Synthetases (NRPSs) and Polyketide Synthases (PKSs) has revealed that it reductively releases 40-phosphopantetheinyl arm-tethered peptidyl product. Article Title: Structural insights into the regulation of NADPH binding to reductase domains of nonribosomal peptide synthetases: A concerted loop movement model. Article Snippet: The termination module of nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) offloads the final product as an acid (occasionally also accompanied by cyclization) upon hydrolysis by employing thioesterase domains (TE-domains).. Reductase domains (R-domains) of short-chain dehydrogenase/reductase (SDR) family offer an alternative offloading mechanism by reducing 4′-phosphopantetheine (4′-PPant) arm-tethered peptidyl chain, a thioester, to an aldehyde or an alcohol.. Recent studies have highlighted their functional importance, for instance in the glycopeptidolipid (GPL) biosynthesis of Mycobacterium smegmatis, where the resulting alcoholic group is the site for subsequent modifications such as glycosylations. Article Title: The heterologous expression of a plastocyanin in the diatom Phaeodactylum tricornutum improves cell growth under iron-deficient conditions. Article Snippet: The PCR product was then cloned into the Article Title: Recombinant expression, purification, and crystallization of the glutaminyl-tRNA synthetase from Toxoplasma gondii. Article Snippet: Aminoacyl tRNA synthetases play a critical role in protein synthesis by providing precursor transfer-RNA molecules correctly charged with their cognate amino-acids.. The essential nature of these enzymes make them attractive targets for designing new drugs against important pathogenic protozoans like Toxoplasma.. Because no structural data currently exists for a protozoan glutaminyl-tRNA synthetase (QRS), an understanding of its potential as a drug target and its function in the assembly of the Toxoplasma multi-aminoacyl tRNA (MARS) complex is therefore lacking. Article Title: Upstream sequence-dependent suppression and AtxA-dependent activation of protective antigens in Bacillus anthracis Article Snippet: To examine the interaction between AtxA and DNA sequences in vitro, the atxA gene was cloned into the expression vector pET 28-b (Novagen, Madison, WI, USA) with N- and C-terminal 6xHIS-tags and an internal FLAG-tag. Article Title: Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs Article Snippet: The LT-A2/LT-B (referred to as LTA2B) fragment from the plasmid pEnt [ ] was PCR amplified and cloned into an N-terminus His 6 tag containing expression vector, Construct:Article Title: DNA-Mediated Signaling by Proteins with 4Fe–4S Clusters Is Necessary for Genomic Integrity Article Snippet: The dinG gene was amplified from E. coli and was inserted into a Article Title: Regulation of gene expression using chromatin remodelling factors Article Snippet: Generation of Article Title: Delineating the reaction mechanism of reductase domains of Nonribosomal Peptide Synthetases from mycobacteria. Article Snippet: Substrate binding to enzymes often follows a precise order where catalysis is accomplished through programmed conformational changes.. Short-chain dehydrogenase/reductase (SDR) enzymes follow sequential order ‘bi–bi’ reaction kinetics.. The mechanistic study of a SDR homolog, reductase (R) domain, from multifunctional enzymes, e.g. Nonribosomal Peptide Synthetases (NRPSs) and Polyketide Synthases (PKSs) has revealed that it reductively releases 40-phosphopantetheinyl arm-tethered peptidyl product. Article Title: Structural insights into the regulation of NADPH binding to reductase domains of nonribosomal peptide synthetases: A concerted loop movement model. Article Snippet: The termination module of nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) offloads the final product as an acid (occasionally also accompanied by cyclization) upon hydrolysis by employing thioesterase domains (TE-domains).. Reductase domains (R-domains) of short-chain dehydrogenase/reductase (SDR) family offer an alternative offloading mechanism by reducing 4′-phosphopantetheine (4′-PPant) arm-tethered peptidyl chain, a thioester, to an aldehyde or an alcohol.. Recent studies have highlighted their functional importance, for instance in the glycopeptidolipid (GPL) biosynthesis of Mycobacterium smegmatis, where the resulting alcoholic group is the site for subsequent modifications such as glycosylations. Article Title: The heterologous expression of a plastocyanin in the diatom Phaeodactylum tricornutum improves cell growth under iron-deficient conditions. Article Snippet: The PCR product was then cloned into the Article Title: Recombinant expression, purification, and crystallization of the glutaminyl-tRNA synthetase from Toxoplasma gondii. Article Snippet: Aminoacyl tRNA synthetases play a critical role in protein synthesis by providing precursor transfer-RNA molecules correctly charged with their cognate amino-acids.. The essential nature of these enzymes make them attractive targets for designing new drugs against important pathogenic protozoans like Toxoplasma.. Because no structural data currently exists for a protozoan glutaminyl-tRNA synthetase (QRS), an understanding of its potential as a drug target and its function in the assembly of the Toxoplasma multi-aminoacyl tRNA (MARS) complex is therefore lacking. Article Title: Upstream sequence-dependent suppression and AtxA-dependent activation of protective antigens in Bacillus anthracis Article Snippet: To examine the interaction between AtxA and DNA sequences in vitro, the atxA gene was cloned into the expression vector pET 28-b (Novagen, Madison, WI, USA) with N- and C-terminal 6xHIS-tags and an internal FLAG-tag. Article Title: Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs Article Snippet: The LT-A2/LT-B (referred to as LTA2B) fragment from the plasmid pEnt [ ] was PCR amplified and cloned into an N-terminus His 6 tag containing expression vector, |